Whole-genome sequencing and antigenic analysis of the first equine influenza virus identified in Turkey.

摘要: BACKGROUND In 2013, there was an outbreak of acute respiratory disease in racehorses Turkey. The clinical signs were consistent with equine influenza (EI). OBJECTIVE aim to confirm the cause and characterise causal virus. METHODS A pan-reactive type real-time RT-PCR a rapid antigen detection kit used for confirmatory diagnosis virus (EIV). Immunological susceptibility EIV examined using single radial haemolysis ELISA. Antigenic characterisation completed by haemagglutinin inhibition panel specific ferret antisera. Genetic achieved whole-genome sequencing segment-specific primers M13 tags. RESULTS H3N8 Florida clade 2 sublineage (FC2) confirmed as agent. index cases unvaccinated immunologically susceptible. Phylogenetic analysis HA1 NA genes demonstrated that A/equine/Ankara/1/2013 clustered FC2 strains circulating Europe. classification absence significant drift. Whole-genome indicated is most closely related viruses described 179 group based on substitution I179V HA1, example A/equine/East Renfrewshire/2/2011, A/equine/Cambremer/1/2012 A/equine/Saone et Loire/1/2015. greatest diversity observed NS1 segment polymerase complex. CONCLUSIONS first recorded EI Turkey caused genetic gave no indication current OIE recommendations vaccine composition require modification.