A genomic approach to bacterial taxonomy: an examination and proposed reclassification of species within the genus Neisseria.

摘要: In common with other bacterial taxa, members of the genus Neisseria are classified using a range phenotypic and biochemical approaches, which not entirely satisfactory in assigning isolates to species groups. Recently, there has been increasing interest nucleotide sequences for typing taxonomy, but date, no broadly accepted alternative conventional methods is available. Here, taxonomic relationships 55 representative have analysed whole-genome sequence data. As genetic material belonging accessory genome widely shared among different taxa present all isolates, this analysis indexed variation within sets genes, specifically protein-coding genes that were directly comparable isolates. Variation these identified seven groups, robust choice phylogenetic clustering used. The groupings largely, completely, congruent current designations, some minor changes nomenclature reassignment few necessary. particular, data showed as polysaccharea polyphyletic probably include more than one taxonomically distinct organism. groups could be reliably rapidly generated 53 ribosomal protein subunit (rps) further demonstrating multilocus (rMLST) practicable powerful means characterizing bacteria at levels, from domain strain.