Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side

摘要: Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first that conditioned media from non-manipulated human embryos cultured vitro 3 days or up to blastocyst stage contain extracellular vesicles (EVs) with diameter of 50 200 nm bearing traditional microvesicle exosome marker proteins CD63, CD9 ALIX. The embryonic origin these EVs has been confirmed by presence stemness gene transcripts their enrichment non-classical HLA-G protein. NANOG POU5F1 were shown be contained deriving at different stages development. In line higher detection rate protein blastocysts compared cleavage embryos, significantly amount was found accumulated spent day 5 development those isolated earlier stage. Uptake dye-labeled embryo-derived primary endometrial epithelial stromal cells also demonstrated fluorescence intensity signal treated derived blastocysts. Based on findings, EV exchange may suggested as an emerging way communication maternal-fetal interface.