Chlamydia Species-Dependent Differences in the Growth Requirement for Lysosomes

摘要: Genome reduction is a hallmark of obligate intracellular pathogens such as Chlamydia, where adaptation to growth has resulted in the elimination genes encoding biosynthetic enzymes. Accordingly, chlamydiae rely heavily on host cell for nutrients yet their specific source unclear. Interestingly, grow within pathogen-defined vacuole that close apposition lysosomes. Metabolically-labeled uninfected proteins were provided an exogenous nutrient chlamydiae-infected cells, and uptake subsequent labeling suggested lysosomal degradation amino acids pathogen. Indeed, Bafilomycin A1 (BafA1), inhibitor vacuolar H+/ATPase blocks acidification functions, impairs C. trachomatis pneumoniae, these effects are especially profound pneumoniae. BafA1 induced marked accumulation material lumen, which was due inhibition proteolytic activities, this response inhibits rather than changes per se, cathepsin inhibitors also inhibit chlamydiae. Finally, addition cycloheximide, eukaryotic protein synthesis, compromises ability block chlamydial growth, suggesting directly access free cytosol preferred nutrients. Thus, co-opt functions lysosomes acquire essential acids.