DNA barcoding Indian marine fishes
作者： W. S. LAKRA , M. S. VERMA , M. GOSWAMI , K. K. LAL , V. MOHINDRA
DOI: 10.1111/J.1755-0998.2010.02894.X
关键词:
摘要: DNA barcoding has been adopted as a global bio-identification system for animals in recent years. A major national programme on of fish and marine life was initiated India by the authors during 2006 115 species covering Carangids, Clupeids, Scombrids, Groupers, Sciaenids, Silverbellies, Mullids, Polynemids Silurids representing 79 Genera 37 Families from Indian Ocean have barcoded first time using cytochrome c oxidase I gene (COI) mtDNA. The were represented multiple specimens total 397 sequences generated. After amplification sequencing 707 base pair fragment COI, primers trimmed which invariably generated 655 barcode sequence. average Kimura two parameter (K2P) distances within species, genera, families, orders 0.30%, 6.60%, 9.91%, 16.00%, respectively. In addition to barcode-based identification system, phylogenetic relationships among also attempted. neighbour-joining tree revealed distinct clusters concurrence with taxonomic status species.
cmfri.org.in参考文章(26)
R. D. Ward, B. H. Holmes, G. K. Yearsley, DNA barcoding reveals a likely second species of Asian sea bass (barramundi) (Lates calcarifer) Journal of Fish Biology. ,vol. 72, pp. 458- 463 ,(2008) , 10.1111/J.1095-8649.2007.01703.X
Nicolas Hubert, Robert Hanner, Erling Holm, Nicholas E. Mandrak, Eric Taylor, Mary Burridge, Douglas Watkinson, Pierre Dumont, Allen Curry, Paul Bentzen, Junbin Zhang, Julien April, Louis Bernatchez, Identifying Canadian Freshwater Fishes through DNA Barcodes PLoS ONE. ,vol. 3, pp. e2490- ,(2008) , 10.1371/JOURNAL.PONE.0002490
Wazir S. Lakra, M. Goswami, A. Gopalakrishnan, Molecular identification and phylogenetic relationships of seven Indian Sciaenids (Pisces: Perciformes, Sciaenidae) based on 16S rRNA and cytochrome c oxidase subunit I mitochondrial genes Molecular Biology Reports. ,vol. 36, pp. 831- 839 ,(2009) , 10.1007/S11033-008-9252-1
Craig Moritz, Carla Cicero, DNA Barcoding: Promise and Pitfalls PLOS Biology. ,vol. 2, ,(2004) , 10.1371/JOURNAL.PBIO.0020354
Paul D. N Hebert, Mark Y Stoeckle, Tyler S Zemlak, Charles M Francis, Identification of Birds through DNA Barcodes PLoS Biology. ,vol. 2, pp. e312- ,(2004) , 10.1371/JOURNAL.PBIO.0020312
Cecilia Saccone, Carla De Giorgi, Carmela Gissi, Graziano Pesole, Aurelio Reyes, Evolutionary genomics in Metazoa: the mitochondrial DNA as a model system. Gene. ,vol. 238, pp. 195- 209 ,(1999) , 10.1016/S0378-1119(99)00270-X
Motoo Kimura, A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution. ,vol. 16, pp. 111- 120 ,(1980) , 10.1007/BF01731581
D E Ruzzante, C T Taggart, D Cook, S Goddard, Genetic differentiation between inshore and offshore Atlantic cod (Gadus morhua) off Newfoundland : microsatellite DNA variation and antifreeze level Canadian Journal of Fisheries and Aquatic Sciences. ,vol. 53, pp. 634- 645 ,(1996) , 10.1139/F95-228
Ian D Hogg, Paul D.N Hebert, Biological identification of springtails (Hexapoda: Collembola) from the Canadian Arctic, using mitochondrial DNA barcodes Canadian Journal of Zoology. ,vol. 82, pp. 749- 754 ,(2004) , 10.1139/Z04-041
J. Thompson, The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research. ,vol. 25, pp. 4876- 4882 ,(1997) , 10.1093/NAR/25.24.4876