Molecular Taxonomy of Serranidae, Subfamily Epinephelinae, Genus Plectropomus (Oken, 1817) of Andaman Waters by DNA Barcoding Using COI Gene Sequence

摘要: Abstract DNA barcoding is an efficient method of biological sample identification and it has been advanced as a promising tool to aid species through use the mitochondrial Cytochrome C Oxidase subunit I (COI) gene standardized region. For variety reasons fishes can be problematic, even for experts, conventional taxonomic studies. So, present study was designed Plectropomus species. Species this highly morphologically ambiguous genus were identified COI sequence analysis. The fish samples collected from seas South Andaman during 2010–11 using standard traditional keys Food Agriculture Organization (FAO). A protocol adopted PCR amplification 650–655 bp genes, which bidirectionally sequenced 20 specimens (mean 6 specimens/species). sequences all interrogated analytical tools available on Barcode Life Data System (BOLD) National Center Biotechnology Information (NCBI) GenBank. All studied showed 99 96 percent genetic identity formed discrete clusters closely related haplogroups. analysis misidentification or misnaming reference P. leopardus pessuliferus. exhibited close relationships with same Australian waters. Further, first documentation This constitutes significant contribution global barcode library demonstrates utility regional identification. more similar maculatus analyzed sequences, distinct distances found between them. It suggested that consideration given revising morphology-based taxonomy checklist view reclassification in Nicobar Islands.