IL-6-inducible complexes on an IL-6 response element of the junB promoter contain Stat3 and 36 kDa CRE-like site binding protein(s).

摘要: The junB gene is one of immediate-early genes whose expression are regulated by a variety extracellular stimuli and play important roles in cellular responses to the given stimuli. Interleukin-6 (IL-6) activates promoter through an IL-6 response element, JRE-IL6, that composed two cooperative DNA motifs, low affinity Stat-binding site overlapping with Ets-binding (JEBS) cAMP responsive element (CRE)-like site. This target for Jak-Stat signal transduction pathway. We showed induced novel complexes on termed JRE-IL6-BC1 2, which contained Stat3 but migrated more slowly than containing homo- or heterodimer Stat1 gel shift assays. These slow-migrating JRE-IL6-BCs appeared contain CRE-like binding proteins besides Stat3, since formation required both JEBS JRE-IL6 oligonucleotides somatostatin CRE efficiently competed making JRE-IL6-BCs. correlated well responsiveness signals. U.v.-cross linking study revealed bound 90 kDa protein, corresponding 36 most likely protein(s). Furthermore, we IL-6/interferon gamma (IFN gamma) IRF-1 (IR/IRF-1), contains adjacent site, also makes IL-6-induced similar