Cloning and Characterization of a Novel Trimethoprim-Resistant Dihydrofolate Reductase from a Nosocomial Isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
作者： Jun-ichiro Sekiguchi , Prasit Tharavichitkul , Tohru Miyoshi-Akiyama , Vena Chupia , Tomoko Fujino
DOI: 10.1128/AAC.49.9.3948-3951.2005
关键词:
摘要: A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that not inhibited by
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