Bio electron microscope and observation method of specimen

摘要: A bio electron microscope and an observation method which can observe a specimen by low damage high contrast to perform high-accuracy image analysis, conduct high-throughput preparation. 1) is observed at accelerating voltage 1.2 4.2 times critical possible transmit obtained under predetermined conditions. 2) An energy filter of small simplified construction provided between the detector for imaging beam in specified region beams transmitting specimen. 3) Similarity such as virus or protein reference known subjected quantitative analysis processing. 4) preparation protocol made into chip using MEMS technique, then mounted on stage part introduction, transfer onto holder.