Regulation of Anabaena sp. strain PCC 7120 glutamine synthetase activity in a Synechocystis sp. strain PCC 6803 derivative strain bearing the Anabaena glnA gene and a mutated host glnA gene.

摘要: The glnA gene from Synechocystis sp. strain PCC 6803 was cloned by hybridization with the Anabaena 7120, and a deletion-insertion mutation of generated in vitro. A derived which contained integrated into chromosome, addition to its own gene, constructed. From that strain, mutant could be obtained transformation inactivated gene; this grew using glutamine synthetase not auxotroph. obtained, however, wild-type enzyme subject ammonium-promoted inactivation when expressed but itself. Images