F plasmid genes involved in the production of recombination-stimulating factor, control of sensitivity to some injurious agents, and chromosome replication in Escherichia coli K-12 HfrC.

摘要: Three related F'arg+ plasmids isolated by Guyer and Clark were used to analyze some properties of strain MG751, a recipient derivative an HfrC mutant (MG7) carrying previously described pleiotropic mutation in the integrated F plasmid. Strains MG7 MG751 both failed produce recombination-stimulating factor, sensitive monofunctional alkylating agents UV irradiation, temperature for growth DNA synthesis. It was shown that these phenotypes are controlled genes (designated rsf, prt, rep) can be separated deletion mutations occurring on