Visual Detection of Transposition of the Maize Element Activator (Ac) in Tobacco Seedlings
作者： J. D. G. JONES , F. M. CARLAND , P. MALIGA , H. K. DOONER
DOI: 10.1126/SCIENCE.244.4901.204
关键词:
摘要: A bacterial streptomycin resistance gene (SPT) was engineered to make it possible detect visually the transposition of maize transposon Activator (Ac) in tobacco. In presence streptomycin, transgenic seedlings carrying SPT appear green, whereas those an SPT:: Ac construct display clones green cells on a white background. Fully arise progeny transformants as result excision before fertilization. About half these germinal revertants carry transposed element. Therefore, constitutes efficient marker for selecting plants that have undergone transposition. maize, there is negative effect increasing dosage frequency and timing This not observed tobacco with variegation assay.
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sciencemag.org参考文章(21)
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