EFFECT OF 60 HZ MAGNETIC FIELD EXPOSURE ON C-FOS EXPRESSION IN STIMULATED PC12 CELLS

摘要: Rat pheochromocytoma PC12 cells have been treated with nerve growth factor (NGF) at final concentrations of 2, 4, 8, and 16 ng/ml, then were exposed to 60-Hz, sinusoidal magnetic fields (MF) 12.5, 25, 50, 100 μT (rms) for 30 min. Transcript levels both c-fos glyceraldehyde-3 -phosphate dehydrogenase determined by Northern blot analysis using 32P-labeled cDNA probes. No change in expression was measured any condition employed. Treatment a combination agents (NGF, forskolin, tetradecanoylphorbol acetate [TPA]) increased over that detected NGF alone. MF exposure the three-agent regimen produced two outcomes, either no or doubling expression. In subsequent experiments, NGF, + forskolin TPA, pre-treated anisomycin TPA. It exposure, like superinduction anisomycin, only cultures which not yet exhibiting maximal is hypothesized may alter activity key intracellular protein kinases.