Specific Associations Between Clinical Signs, Immune Cells, Disease Genetic Background and Burden in a Group of Patients with B-Cell Chronic Lymphocytic Leukemia

摘要: Traffic of tumorand normal cells through the peripheral blood (PB) patients with B-cell chronic lymphocytic leukemia (B-CLL) to lymph nodes (LN) or spleen/ liver sites is governed by specific changes in surface and intracellular molecule expression. The study aims investigate potential association between different lymphocyte subsets, chemokine receptors genetic alterations clinical signs a group B-CLL patients. Forty-three were included study. expression CCR7, CXCR5, CXCR3, CCR4, CD3, CD4, CD8, CD27, CD28, CD45RA, CD25, CD127, CD38 was tested multiparameter flow cytometry. Genetic determined MLPA. We found increased frequency CD38+ directly correlated presence LN>5cm. CXCR5 CCR7 are homogenously expressed monoclonal cells. CCR4+ cell be lower PB presenting particular LN involvement. Heterogeneous complex only trisomy 12 associated less frequent axillary also report significant increase total T subsets (effectorand central memory CD4+ cells, regulatory follicular helper distinct functional CD8+ cells) occurrence manifestations. Chemokine receptor on circulating augmented connection some locations. Consequently, manifestations linked both, factors intrinsic B external influences coming from microenvironment.