Distinctive patterns of naïve/memory subset distribution and cytokine expression in CD4 T lymphocytes in ZAP-70 B-chronic lymphocytic patients.

摘要: Background ZAP-70 upregulation in B chronic lymphocytic leukemia (B-CLL) cells is a recognized marker of poor prognosis these patients; the biological basis this differential clinical outcome nonetheless remains unknown. ZAP-70 overexpression considered surrogate B-CLL cell subset. To test whether characteristics patients also include T helper population, we studied naive, non-terminated memory (NTEM), terminated (TEM) and central (CM) cells, cytokine expression by CD4 lymphocytes from ZAP-70+ ZAP-70− patients. Methods Expression CD3, CD8, CD45RA, CD27, CD28 antigens intracytoplasmic production (IFNγ, IL-2, IL-4, IL-10, TNFα) were assessed simultaneously nine-color flow-cytometry peripheral blood patients. was analyzed flow cytometry. Results Compared to patients, showed 1) significant reduction naive subset expansion NTEM TEM subsets, 2) decrease percentage activated expressing IFNγ, TNFα, 3) an increase IL-4 or IL-10. Conclusions In conclusion, early stage associated with distinct patterns activation/differentiation distribution lymphocytes. © 2013 International Clinical Cytometry Society