Structural Model for the Spider Silk Protein Spidroin-1

摘要: Most reports about the 3-D structure of spidroin-1 have been proposed for protein in solid state or individual domains these proteins. A gel-based mass spectrometry strategy using collision-induced dissociation (CID) and electron-transfer (ETD) fragmentation methods was used to completely sequence spidroins-1A -1B assign a series post-translational modifications (PTMs) on spidroin sequences. total 15 16 phosphorylation sites were detected spidroin-1A -1B, respectively. In this work, we present nearly complete amino acid including nonrepetitive N- C-terminal highly repetitive central core. We also described fatty layer surrounding fibers PTMs sequences phosphorylation. Thus, molecular models phosphorylated spidroins presence mixture acids/water (1:1) submitted dynamics simu...