Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines

摘要: Primary cilia (PC) were demonstrated for the first time in fish endothelial, epithelial and fibroblast cell lines through immunofluorescence staining with monoclonal antibody, 6-11B-1, against acetylated α-tubulin. The study was carried out eight recently developed from walleye, Sander vitreus (Mitchill). These three fibroblast-like lines, WE-cfin11f, WE-skin11f WE-liver3 from, respectively, caudal fin, skin liver, epithelial-like WE-cfin11e, WE-spleen6 WErpe spleen retina. Also, endothelial-like WEBA bulbus arteriosus glial-like WE-brain5 brain used. Immunocytochemistry revealed strong α-tubulin mitotic spindles midbodies all PC except WE-skin11f. Staining of cytoplasmic microtubules (fibrils) absent including WEBA, but present others, especially WE-skin11f, which might have obscured detection these cells. Tubacin, an inhibitor histone deacetylase 6, induced fibrils intensity their WE-cfin11f. results suggest that differ activities, making them useful studying this tubulin modification teleosts, as well PC.