Second generation sequencing of microRNA in Human Bone Cells treated with Parathyroid Hormone or Dexamethasone
作者： Navya Laxman , Carl-Johan Rubin , Hans Mallmin , Olle Nilsson , Christian Tellgren-Roth
DOI: 10.1016/J.BONE.2015.12.053
关键词:
摘要: We investigated the impact of treatment with parathyroid hormone (PTH) and dexamethasone (DEX) for 2 24h by RNA sequencing miRNAs in primary human bone (HOB) cells. A total 207 million reads were obtained, normalized absolute expression retrieved 373 most abundant miRNAs. In naive control cells, 7 differentially expressed (FDR<0.05) between two time points. Ten exhibited differential (FDR <0.05) across points treatments after adjusting controls selected downstream analyses. Results show significant effects on miRNA when comparing PTH DEX at 2h even more pronounced 24h. Interestingly, several exhibiting differences are predicted to target genes involved metabolism e.g. miR-30c2, miR-203 miR-205 targeting RUNX2, miR-320 β-catenin (CTNNB1) mRNA expression. CTNNB1and RUNX2 levels decreased increased treatment. Our analysis also identified putative novel treated cells showed that affect HOB regulated turn correlated key metabolism.
elsevier.com
sci-hub.se 参考文章(45)
Jee Hyun An, Jung Hun Ohn, Jung Ah Song, Jae-Yeon Yang, Hyojung Park, Hyung Jin Choi, Sang Wan Kim, Seong Yeon Kim, Woog-Yang Park, Chan Soo Shin, Changes of MicroRNA Profile and MicroRNA‐mRNA Regulatory Network in Bones of Ovariectomized Mice Journal of Bone and Mineral Research. ,vol. 29, pp. 644- 656 ,(2014) , 10.1002/JBMR.2060
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
Theodora Koromila, Sanjeev K. Baniwal, Yae S. Song, Anthony Martin, Jian Xiong, Baruch Frenkel, Glucocorticoids Antagonize RUNX2 During Osteoblast Differentiation in Cultures of ST2 Pluripotent Mesenchymal Cells Journal of Cellular Biochemistry. ,vol. 115, pp. 27- 33 ,(2014) , 10.1002/JCB.24646
Tao Li, Hongling Li, Tangping Li, Junfen Fan, Robert Chunhua Zhao, Xisheng Weng, MicroRNA expression profile of dexamethasone-induced human bone marrow-derived mesenchymal stem cells during osteogenic differentiation. Journal of Cellular Biochemistry. ,vol. 115, pp. 1683- 1691 ,(2014) , 10.1002/JCB.24831
Lee P Lim, Margaret E Glasner, Soraya Yekta, Christopher B Burge, David P Bartel, Vertebrate microRNA genes. Science. ,vol. 299, pp. 1540- 1540 ,(2003) , 10.1126/SCIENCE.1080372
Elin Grundberg, Veronique Adoue, Tony Kwan, Bing Ge, Qing Ling Duan, Kevin C. L. Lam, Vonda Koka, Andreas Kindmark, Scott T. Weiss, Kelan Tantisira, Hans Mallmin, Benjamin A. Raby, Olle Nilsson, Tomi Pastinen, Global Analysis of the Impact of Environmental Perturbation on cis-Regulation of Gene Expression PLoS Genetics. ,vol. 7, pp. e1001279- ,(2011) , 10.1371/JOURNAL.PGEN.1001279
Yi-Chih Sun, Hui-Chen Huang, Feng-Sheng Wang, Chun-Liang Lin, Yeung-Jen Chen, Ching-Jen Wang, Kuender D. Yang, Yu-Ting Huang, Secreted frizzled-related protein 1 modulates glucocorticoid attenuation of osteogenic activities and bone mass. Endocrinology. ,vol. 146, pp. 2415- 2423 ,(2005) , 10.1210/EN.2004-1050
D. Patschan, K. Loddenkemper, F. Buttgereit, Molecular mechanisms of glucocorticoid-induced osteoporosis. Bone. ,vol. 29, pp. 498- 505 ,(2001) , 10.1016/S8756-3282(01)00610-X
Zhaoyong Li, Mohammad Q Hassan, Mohammed Jafferji, Rami I Aqeilan, Ramiro Garzon, Carlo M Croce, Andre J van Wijnen, Janet L Stein, Gary S Stein, Jane B Lian, None, Biological Functions of miR-29b Contribute to Positive Regulation of Osteoblast Differentiation Journal of Biological Chemistry. ,vol. 284, pp. 15676- 15684 ,(2009) , 10.1074/JBC.M809787200
Roland Baron, Michaela Kneissel, WNT signaling in bone homeostasis and disease: from human mutations to treatments Nature Medicine. ,vol. 19, pp. 179- 192 ,(2013) , 10.1038/NM.3074