An in vitro model for hypertrophic adipocytes: Time-dependent adipocyte proteome and secretome changes under high glucose and high insulin conditions.

摘要: Obesity is the consequence of a positive energy balance and characterized by enlargement adipose tissue, which in part due to hyperplasia hypertrophy adipocytes. Not much known about transition normal mature adipocytes hypertrophic state, vivo very hard study. Here, we have maintained human SGBS cells as surrogate for adipocytes, changes morphological molecular metabolism were monitored over first 4 days last days. In total, 393 cellular proteins 246 secreted identified further analysis. During high glucose insulin, seemed prefer pyruvate source, whereas beta-oxidation was down-regulated supporting lipid loading. Over time, droplet fusion instead uptake became relatively important growth droplets during Moreover, ECM production shifted towards turnover up-regulation proteases eight The present vitro system provides insight into metabolic under conditions may help understand process adipocyte development obesity.