Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA

摘要: Dnmt3a is a de novo DNA methyltransferase that modifies unmethylated DNA. In contrast Dnmt1 shows high preference for hemimethylated However, can be activated the methylation of unmodified We show here and methyltransferases functionally cooperate in DNA, because fivefold stimulation activity observed if both enzymes are present. Stimulation used before Dnmt1, but not incubation with precedes Dnmt3a, demonstrating by stimulates no physical interaction required. If were incubated together slightly increased could due to direct these enzymes. addition, we stimulated already carries some methyl groups. conclude after initiation becomes pre-existing groups further methylates Our data suggest also has role This model agrees biochemical properties provides mechanistic basis functional cooperation different MTases been vivo.