Aurora-A Contributes to Radioresistance by Increasing NF-κB DNA Binding
作者： Eun-Taex Oh , Mi-Sun Byun , Hyemi Lee , Moon-Taek Park , Dae-Myung Jue
DOI: 10.1667/RR2017.1
关键词:
摘要: Abstract Aurora-A, a serine/threonine kinase that is overexpressed in certain human cancer cell lines, plays an important role mitotic progression. Aurora-A has also been reported to be involved the activation of nuclear factor kappa B (NF-κB). The purpose present study was identify radiation-induced pathway NF-κB. Wild-type and knockdown (Aurora-AKD) HeLa cells were irradiated with 4 Gy γ rays EMSA, luciferase reporter gene assay immunoblot analysis performed. siRNA-based overexpression system adopted elucidate NF-κB activation. clonogenic survival indicated Aurora-AKD wild-type transfected siRNA or RelA/p65 more radiosensitive than cells. In both cells, radiation caused IκB kinase-mediated phosphorylation, degradation IκBα phosphor...
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