Basic fibroblast growth factor promotes stem Leydig cell development and inhibits LH-stimulated androgen production by regulating microRNA expression.
作者： Hui Liu , Yan Yang , Lei Zhang , Rui Liang , Ren-shan Ge
DOI: 10.1016/J.JSBMB.2014.09.016
关键词:
摘要: Leydig cells are the primary source of testosterone in testes, and their steroidogenic function is strictly controlled by hypothalamus-pituitary-gonad axis. Emerging evidence has indicated that fibroblast growth factors play a role regulating stem cell development steroidogenesis, but little known about regulatory mechanism. Using seminiferous tubule culture system, we demonstrated basic factor (bFGF) can promote proliferation commitment toward differentiation testosterone-producing cells. However, these promoting effects decreased with an increase bFGF dose. Previous studies have reported inhibits luteinizing hormone (LH)-stimulated androgen production downregulating mRNA expression genes immature levels 677 microRNAs did not change significantly during LH-mediated process synthesis. Five (miR-29a, -29c, -142-3p, -451 -335) were identified, was regulated simultaneously LH. These results suggested inhibition LH-stimulated may be modulated bFGF-mediated microRNA expression, which further impacts signaling pathway biosynthesis gene expression.
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