Overexpression of Heparin-Binding Growth-Associated Molecule in Malignant Glioma Cells
作者： Lei Zhang , Tadashi Mabuchi , Eiji Satoh , Shuichiro Maeda , Hideaki Nukui
DOI: 10.2176/NMC.44.637
关键词:
摘要: The highly invasive and angiogenic characteristics of malignant gliomas depend on the production growth factors factors. Heparin-binding growth-associated molecule (HB-GAM) is a secreted factor that mitogenic for endothelial cells. To examine expression profile HB-GAM in glioma cells, messenger ribonucleic acid (mRNA) was analyzed 10 cell lines, two glioblastoma tissue specimens, normal brain specimens by reverse transcription-polymerase chain reaction. mRNA expressed all including specimens. Western blot analysis revealed protein contents lines tissues were 1.8 to 6.3 times higher than those tissues. effect neutralizing anti-platelet-derived (PDGF) antibody also examined since cells secrete PDGF upregulates expression. Treatment U251 T98G with anti-PDGF did not affect production. These results suggest overexpressed involved tumor growth.
sci-hub.se 参考文章(41)
Sylvia A. Norman, John G. Golfinos, Adrienne C. Scheck, Expression of a receptor protein tyrosine phosphatase in human glial tumors. Journal of Neuro-oncology. ,vol. 36, pp. 209- 217 ,(1998) , 10.1023/A:1005840420136
H. Rauvala, An 18-kd heparin-binding protein of developing brain that is distinct from fibroblast growth factors. The EMBO Journal. ,vol. 8, pp. 2933- 2941 ,(1989) , 10.1002/J.1460-2075.1989.TB08443.X
Garrett M. Brodeur, Jeffrey Milbrandt, Huaqing Zhao, Akira Nakagawara, Thomas F. Deuel, Takashi Muramatsu, Avital Cnaan, Differential Expression of Pleiotrophin and Midkine in Advanced Neuroblastomas Cancer Research. ,vol. 55, pp. 1792- 1797 ,(1995)
A Wellstein, W J Fang, A Khatri, Y Lu, S S Swain, R B Dickson, J Sasse, A T Riegel, M E Lippman, A heparin-binding growth factor secreted from breast cancer cells homologous to a developmentally regulated cytokine. Journal of Biological Chemistry. ,vol. 267, pp. 2582- 2587 ,(1992) , 10.1016/S0021-9258(18)45920-0
E. Grouzmann, E. Van Meir, A. Fontana, F. Effenberger, N. De Tribolet, I. Desbaillets, A. Walz, K. Frei, M. Ceska, Interleukin-8 is produced in neoplastic and infectious diseases of the human central nervous system. Cancer Research. ,vol. 52, pp. 4297- 4305 ,(1992)
J Merenmies, H Rauvala, Molecular cloning of the 18-kDa growth-associated protein of developing brain. Journal of Biological Chemistry. ,vol. 265, pp. 16721- 16724 ,(1990) , 10.1016/S0021-9258(17)44817-4
Y.S. Li, R.M. Hoffman, M.M. Le Beau, R Espinosa, N.A. Jenkins, D.J. Gilbert, N.G. Copeland, T.F. Deuel, Characterization of the human pleiotrophin gene. Promoter region and chromosomal localization. Journal of Biological Chemistry. ,vol. 267, pp. 26011- 26016 ,(1992) , 10.1016/S0021-9258(18)35709-0
Rolf Mentlein, Janka Held-Feindt, Pleiotrophin, an angiogenic and mitogenic growth factor, is expressed in human gliomas Journal of Neurochemistry. ,vol. 83, pp. 747- 753 ,(2002) , 10.1046/J.1471-4159.2002.01179.X
F Czubayko, A Wellstein, A T Riegel, Ribozyme-targeting elucidates a direct role of pleiotrophin in tumor growth. Journal of Biological Chemistry. ,vol. 269, pp. 21358- 21363 ,(1994) , 10.1016/S0021-9258(17)31970-1
W Fang, N Hartmann, D.T. Chow, A.T. Riegel, A Wellstein, Pleiotrophin stimulates fibroblasts and endothelial and epithelial cells and is expressed in human cancer Journal of Biological Chemistry. ,vol. 267, pp. 25889- 25897 ,(1992) , 10.1016/S0021-9258(18)35692-8