Electron transport components localized in a lipid-depleted sheet isolated from Micrococcuslysodeikticus membranes by deoxycholate extraction

摘要: Abstract Extraction of isolated membranes from Micrococcus lysodeikticus with 1% deoxycholate in 0.05M tris buffer yields an insoluble residue which accounts for 15% the dry weight membrane. This fraction is composed largely protein and contains 3–5% residual lipid compared to 23–26% initial membrane consequently has a higher density than on gradient centrifugation. These lipid-depleted residues are form membranous sheet when examined electron microscope. Cytochromes a, b c succinic dehydrogenase activity were localized almost exclusively this