ORIGINAL ARTICLE: Effect of Progesterone on Proinflammatory Cytokine Production by Monocytes Stimulated with Pathogens Associated with Preterm birth

摘要: PROBLEM A number of clinical trials have demonstrated that supplemental progesterone (P4) can prevent preterm birth. Although P4 decrease the production mediators inflammation by lipopolysaccharide (LPS)-stimulated macrophages, a majority infections associated with birth are due to Ureaplasma urealyticum, which does not contain LPS. Therefore, we studied whether could lower proinflammatory cytokines monocytes stimulated U. urealyticum. METHOD OF STUDY Human (THP-1 cells) were treated and then heat-killed Escherichia coli or Cytokine concentrations in conditioned medium measured ELISA relative viability cells was assessed colorimetrically. The impact on interleukin (IL)-1beta, tumor necrosis factor-alpha (TNF-alpha) IL-8 comparing levels across different dosages organism concentrations. RESULTS Both organisms increased IL-1alpha, TNF-alpha dose-dependent manner. enhanced IL-1beta IL-8, but inhibited either organism. CONCLUSION modulates cytokine E. urealyticum-stimulated similar manner is strictly immunosuppressive. This suggests simply suppressing bacteria-stimulated production.