In vivo localization of human colon adenocarcinoma by monoclonal antibody binding to a highly expressed cell surface antigen.

摘要: We have produced a monoclonal antibody (SF-25) against human hepatoma cell line (FOCUS) that strongly reacts with an antigen shared by all six colon adenocarcinoma lines. This surface was uniformly expressed in 17 adenocarcinomas of the obtained at surgery but not on normal adjacent mucosa counterpart. Other tissues were negative except for population cells distal tubule kidney as shown immunoperoxidase staining and direct binding to membrane preparations. Binding this M r 125,000 is disrupted detergents, sodium dodecyl sulfate, paraformaldehyde fixation treatment FOCUS trypsin. The SF-25 when labeled 125I shows striking capacity both biodistribution nuclear imaging studies localize grown solid tumors nude mice. may be useful distinguishing between transformed phenotype.