Poly(ethylene glycol)-Lipid-Conjugated Antibodies Enhance Dendritic Cell Phagocytosis of Apoptotic Cancer Cells

摘要: A simple method for attaching immunoglobulin G (IgG) on the cell surface was successfully developed enhancing phagocytosis of apoptotic tumor cells (ATCs) by dendritic (DCs) ex vivo. By conjugating with a poly(ethylene glycol) (PEG)-lipid, named biocompatible anchor membrane (BAM), arbitrary IgG could be incorporated into membrane. In particular, when IgG-BAM conjugates were prepared at optimal molar ratio to BAM (1 20), almost all efficiently modified treatment IgG-BAM. This applied four types mammalian cells. Furthermore, ATCs conjugate increased DCs two-fold compared no treatment. phagocytosis-enhancing effect nearly identical tumor-specific IgG. Thus, without employing IgG, which is difficult obtain any and expensive, present opsonize ATC use The results strongly indicate that represents promising opsonizing human serum this approach will lead objective clinical responses in DC vaccines.