Proteomic analysis of the response of α-ketoglutarate-producer Yarrowia lipolytica WSH-Z06 to environmental pH stimuli

摘要: During bioproduction of short-chain carboxylates, a shift in pH is common strategy for enhancing the biosynthesis target products. Based on two-dimensional gel electrophoresis, comparative proteomics analysis general and mitochondrial protein samples was used to investigate cellular responses environmental stimuli α-ketoglutarate overproducer Yarrowia lipolytica WSH-Z06. The lower tensioned intracellular acidification increased level reactive oxygen species (ROS). A total 54 differentially expressed spots were detected, 11 main processes identified be involved response stimuli. Slight decrease cytoplasmic enhanced acidogenicity by elevating expression key enzymes tricarboxylic acid cycle (TCA cycle). Enhanced energy biosynthesis, ROS elimination, membrane potential homeostasis also employed as defense strategies compete with Owing its antioxidant role α-ketoglutarate, metabolic flux shifted under Y. acidic proteins provide clues understanding mechanisms carboxylate production through engineering or process optimization combination manipulation conditions.