The Interaction of Ribonuclease with Metal Ions II. GEL FILTRATION STUDIES OF CUPRIC ION-RIBONUCLEASE INTERACTIONS

作者: Esther Breslow , Albert W. Girotti

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摘要: Abstract Gel filtration studies indicate that ribonuclease has five cupric ion-binding sites in the pH region 5.5 to 7.0. At 5.5, there are one "strong" and four weaker sites, all of which can bind both free ion copper-monoacetate complex. 7, distribution among is acetate-dependent. Affinities different for ions given.

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