Original Article Regulation of apoptosis by long non-coding RNA HIF1A-AS1 in VSMCs: implications for TAA pathogenesis

作者： Yanzhi Wang , Yuehong Yue , Guangxing Feng , Yongbo Zhao , Weichao Zhao

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摘要: Objective: Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as transcriptional regulation, cell growth and tumorigenesis. However, little was known about whether lncRNA HIF 1 alpha-antisense RNA (HIF1a-AS1) regulating the proliferation apoptosis of VSMCs vitro expres- sion HIF1a-AS1 serum TAA patients. Methods: The viability detected by CCK8 assay. assessed annexin V-PI double-labeling staining. Expression genes proteins were analyzed real-time PCR western blotting respectively. Cells transfected with siRNAs a gene silencing methods. Results: In patients, expression significantly increased (superior to 6 folds) compared normal control. Moreover, PA induced time- dose-dependent manner, proportion apoptotic cells had gained untreatment group. also upregulation HIF1a-AS1. We found that transfection siRNA decreased caspase3 caspase8 Bcl2, protected PA-induced VSMCs. Conclusions: overexpressed thoracoabdominal aorta aneurysm interaction between plays key role vitro, which may contribute pathogenesis aneurysm.

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参考文章(24)

J. Zanow, U. Settmacher, Aneurysmen der thorakalen und thorakoabdominalen Aorta Chirurg. ,vol. 85, pp. 767- 773 ,(2014) , 10.1007/S00104-014-2717-Y

Nakwon Choe, Jin-Sook Kwon, Ju-Ryoung Kim, Gwang Hyeon Eom, Yongsook Kim, Kwang-Il Nam, Youngkeun Ahn, Hae Jin Kee, Hyun Kook, The microRNA miR-132 targets Lrrfip1 to block vascular smooth muscle cell proliferation and neointimal hyperplasia. Atherosclerosis. ,vol. 229, pp. 348- 355 ,(2013) , 10.1016/J.ATHEROSCLEROSIS.2013.05.009

Joseph Bobadilla, Nathan Orr, David Minion, Thoracoabdominal aortic aneurysm repair: current endovascular perspectives. Vascular Health and Risk Management. ,vol. 10, pp. 493- 505 ,(2014) , 10.2147/VHRM.S46452

Tony Gutschner, Sven Diederichs, The hallmarks of cancer: a long non-coding RNA point of view. RNA Biology. ,vol. 9, pp. 703- 719 ,(2012) , 10.4161/RNA.20481

Ci Chu, Kun Qu, Franklin L. Zhong, Steven E. Artandi, Howard Y. Chang, Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions. Molecular Cell. ,vol. 44, pp. 667- 678 ,(2011) , 10.1016/J.MOLCEL.2011.08.027

Elena S. Martens-Uzunova, René Böttcher, Carlo M. Croce, Guido Jenster, Tapio Visakorpi, George A. Calin, Long Noncoding RNA in Prostate, Bladder, and Kidney Cancer European Urology. ,vol. 65, pp. 1140- 1151 ,(2014) , 10.1016/J.EURURO.2013.12.003

Khelifa Arab, Yoon Jung Park, Anders M. Lindroth, Andrea Schäfer, Christopher Oakes, Dieter Weichenhan, Annekatrin Lukanova, Eva Lundin, Angela Risch, Michael Meister, Hendrik Dienemann, Gerhard Dyckhoff, Christel Herold-Mende, Ingrid Grummt, Christof Niehrs, Christoph Plass, Long Noncoding RNA TARID Directs Demethylation and Activation of the Tumor Suppressor TCF21 via GADD45A Molecular Cell. ,vol. 55, pp. 604- 614 ,(2014) , 10.1016/J.MOLCEL.2014.06.031

Liqun Jiang, Mingyi Wang, Jing Zhang, Robert E. Monticone, Richard Telljohann, Gaia Spinetti, Gianfranco Pintus, Edward G. Lakatta, Increased Aortic Calpain-1 Activity Mediates Age-Associated Angiotensin II Signaling of Vascular Smooth Muscle Cells PLoS ONE. ,vol. 3, pp. e2231- ,(2008) , 10.1371/JOURNAL.PONE.0002231

Vincent L. Rowe, Scott L. Stevens, Tonya T. Reddick, Michael B. Freeman, Robert Donnell, Roger C. Carroll, Mitchell H. Goldman, Vascular smooth muscle cell apoptosis in aneurysmal, occlusive, and normal human aortas. Journal of Vascular Surgery. ,vol. 31, pp. 567- 576 ,(2000) , 10.1067/MVA.2000.102847

10.

Noriyuki Yajima, Masahisa Masuda, Masaru Miyazaki, Nobuyuki Nakajima, Shu Chien, John YJ Shyy, None, Oxidative stress is involved in the development of experimental abdominal aortic aneurysm: A study of the transcription profile with complementary DNA microarray Journal of Vascular Surgery. ,vol. 36, pp. 379- 385 ,(2002) , 10.1067/MVA.2002.124366

Original Article Regulation of apoptosis by long non-coding RNA HIF1A-AS1 in VSMCs: implications for TAA pathogenesis

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Original Article Regulation of apoptosis by long non-coding RNA HIF1A-AS1 in VSMCs: implications for TAA pathogenesis

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