Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens.
作者： R L Evans , T J Faldetta , R E Humphreys , D M Pratt , E J Yunis
关键词:
摘要: We have studied the modulation of Ia-like antigens on surface membrane human T cells responding in a one-way mixed leukocyte culture. A heterologous antiserum, (anti-p23,30), which is specific to HLA-D-related and unreactive with normal peripheral or thymocytes, was found bind significantly all transformed culture (MLC) as determined by indirect immunofluorescence fluorescence-activated cell sorter 1. Furthermore, cytotoxic responsible for cell-mediated lympholysis were shown react anti-p23,30, whereas their unactivated progenitors did not. Immunoprecipitation sodium dodecyl sulfate polyacrylamide gel electrophoresis radioactive 29,000 34,000 dalton complex from MLC-primed labeled [35S]methionine indicated that allosensitized synthesized these antigens.
rupress.org
jem.org参考文章(15)
M J Turner, P Cresswell, P Parham, J L Strominger, D L Mann, A R Sanderson, Purification of papain-solubilized histocompatibility antigens from a cultured human lymphoblastoid line, RPMI 4265. Journal of Biological Chemistry. ,vol. 250, pp. 4512- 4519 ,(1975) , 10.1016/S0021-9258(19)41332-X
Stuart F. Schlossman, Ann C. Penta, Herbert Lazarus, Robert L. Evans, Two functionally distinct subpopulations of human T cells that collaborate in the generation of cytotoxic cells responsible for cell-mediated lympholysis. Journal of Immunology. ,vol. 120, pp. 1423- 1428 ,(1978)
Steven W. Kessler, Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology. ,vol. 115, pp. 1617- 1624 ,(1975)
S. F. Schlossman, L. Chess, R. E. Humphreys, J. L. Strominger, Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 73, pp. 1288- 1292 ,(1976) , 10.1073/PNAS.73.4.1288
M. J. Giphart, J. F. Kaufman, A. Fuks, D. Albrechtsen, B. G. Solheim, J. W. Bruning, J. L. Strominger, HLA-D associated alloantisera react with molecules similar to Ia antigens. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 3533- 3536 ,(1977) , 10.1073/PNAS.74.8.3533
Z Nagy, B E Elliott, M Nabholz, P H Krammer, B Pernis, Specific binding of alloantigens to T cells activated in the mixed lymphocyte reaction. Journal of Experimental Medicine. ,vol. 143, pp. 648- 659 ,(1976) , 10.1084/JEM.143.3.648
Jonathan King, Ulrich K. Laemmli, Polypeptides of the tail fibres of bacteriophage T4 Journal of Molecular Biology. ,vol. 62, pp. 465- 477 ,(1971) , 10.1016/0022-2836(71)90148-3
R E Humphreys, J M McCune, L Chess, H C Herrman, D J Malenka, D L Mann, P Parham, S F Schlossman, J L Strominger, Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen. Journal of Experimental Medicine. ,vol. 144, pp. 98- 112 ,(1976) , 10.1084/JEM.144.1.98
T Tada, M Taniguchi, C S David, Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex. Journal of Experimental Medicine. ,vol. 144, pp. 713- 725 ,(1976) , 10.1084/JEM.144.3.713
M J Taussig, A J Munro, R Campbell, C S David, N A Staines, Antigen-specific T-cell factor in cell cooperation. Mapping within the I region of the H-2 complex and ability to cooperate across allogeneic barriers. Journal of Experimental Medicine. ,vol. 142, pp. 694- 700 ,(1975) , 10.1084/JEM.142.3.694