Two functionally distinct subpopulations of human T cells that collaborate in the generation of cytotoxic cells responsible for cell-mediated lympholysis.

摘要: A human thymus-dependent differentiation antigen, TH2 was defined by a rabbit anti-human T cell serum absorbed with autologous B lymphoblasts and leukemic cells bearing markers from patient chronic lymphocytic leukemia. Anti-TH2 reacted specifically thymus-derived lymphoid exhibited two distinct profiles of reactivity normal peripheral as detected indirect immunofluorescence on FACS I. Isolation strongly reactive, TH2+, weakly TH2- fluorescence-activated sorting revealed that the TH2+ subset contained most killer activity in cell-mediated lympholysis (CML), but had diminished response MLC suboptimal or negligible proliferative to soluble antigens (mumps, PPD, tetanus toxoid). In contrast, markedly less amplified cytotoxicity both alloantigen often significantly greater than unseparated cells. The relevance these findings previously described subsets functional subpopulations murine is discussed.