Enrichment for CFU-C from murine and human bone marrow using soybean agglutinin.
作者： Y Reisner , N Kapoor , MZ Hodes , RJ O'Reilly , RA Good
DOI: 10.1182/BLOOD.V59.2.360.360
关键词:
摘要: Mouse bone marrow and spleen cells agglutinated by soybean agglutinin (SBA) or peanut (PNA) were previously shown to be enriched for colony-forming (CFU-S) sufficiently depleted of graft-versus-host reaction producing allow hematologic reconstitution lethally irradiated allogeneic recipient mice. A similar enrichment capable forming colonies in soft agar culture (CFU-C) has now been found the SBA-agglutinated fraction mouse cells, contrast finding that human majority CFU-C are not SBA. Cytofluorometric studies with fluorescein-labeled SBA (FITC-SBA) revealed both bind lectin. Experiments mixing fractions separated reveal true is achieved unagglutinated fraction, as opposed a possible depletion suppressor cell population. Granulocytic, monocytic, mixed all SBA-unagglutinated from marrow.
ashpublications.org
sci-hub.se 参考文章(12)
Stuart F. Schlossman, Ann C. Penta, Herbert Lazarus, Robert L. Evans, Two functionally distinct subpopulations of human T cells that collaborate in the generation of cytotoxic cells responsible for cell-mediated lympholysis. Journal of Immunology. ,vol. 120, pp. 1423- 1428 ,(1978)
E. DONNALL THOMAS, RAINER STORB, Technique for Human Marrow Grafting Blood. ,vol. 36, pp. 507- 515 ,(1970) , 10.1182/BLOOD.V36.4.507.507
Beverley L. Pike, William A. Robinson, Human bone marrow colony growth in agar-gel. Journal of Cellular Physiology. ,vol. 76, pp. 77- 84 ,(1970) , 10.1002/JCP.1040760111
Y. Reisner, S. Pahwa, J. W. Chiao, N. Sharon, R. L. Evans, R. A. Good, Separation of antibody helper and antibody suppressor human T cells by using soybean agglutinin Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 6778- 6782 ,(1980) , 10.1073/PNAS.77.11.6778
Yair Reisner, Amiram Ravid, Nathan Sharon, Use of soybean agglutinin for the separation of mouse B and T lymphocytes. Biochemical and Biophysical Research Communications. ,vol. 72, pp. 1585- 1591 ,(1976) , 10.1016/S0006-291X(76)80195-7
Y. Reisner, L. Itzicovitch, A. Meshorer, N. Sharon, Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 2933- 2936 ,(1978) , 10.1073/PNAS.75.6.2933
Yair Reisner, RichardJ. O'Reilly, Neena Kapoor, RobertA. Good, Allogeneic bone marrow transplantation using stem cells fractionated by lectins: VI, in vitro analysis of human and monkey bone marrow cells fractionated by sheep red blood cells and soybean agglutinin. The Lancet. ,vol. 316, pp. 1320- 1324 ,(1980) , 10.1016/S0140-6736(80)92394-6
Y. Reisner, S. Ikehara, M. Z. Hodes, R. A. Good, Allogeneic hemopoietic stem cell transplantation using mouse spleen cells fractionated by lectins: in vitro study of cell fractions Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 1164- 1168 ,(1980) , 10.1073/PNAS.77.2.1164
Nicos A. Nicola, Donald Metcalf, Gregory R. Johnson, Antony W. Burgess, Preparation of colony stimulating factors from human placental conditioned medium Leukemia Research. ,vol. 2, pp. 313- 322 ,(1978) , 10.1016/0145-2126(78)90018-8
D. Metcalf, H. R. Macdonald, Heterogeneity of in vitro colony- and cluster-forming cells in the mouse marrow: Segregation by velocity sedimentation Journal of Cellular Physiology. ,vol. 85, pp. 643- 653 ,(1975) , 10.1002/JCP.1040850317