A reappraisal of the role of Zn2+ in TGF-β1-induced apoptosis in primary hepatocytes
作者： S.H. Inayat-Hussain , G.M. Cohen , K. Cain
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摘要: There is now a wealth of information regarding the apoptotic mode cell death and its importance in toxicological studies many mammalian organs including liver. In this study, we investigated modulatory effects heavy metal Zn2+ on transforming growth factor-beta1 (TGF-beta1)-induced apoptosis primary rat hepatocytes. Apoptosis induced by TGF-beta1 (1 ng/ml) hepatocytes was accompanied nuclear condensation as assessed morphologically staining with Hoechst 33258 DNA cleavage detected biochemically situ end-labeling, field inversion conventional gel electrophoresis. Pretreatment 100 micromol/L abrogated condensation, laddering TGF-beta1-treated Surprisingly, did not inhibit formation high-molecular-weight fragments (30-50 kbp to 250-300 kbp). These data provide evidence that exerts endonucleases act downstream execution phase TGF-beta1-induced
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sci-hub.se 参考文章(36)
J. Filipski, J. Leblanc, T. Youdale, M. Sikorska, P.R. Walker, Periodicity of DNA folding in higher order chromatin structures. The EMBO Journal. ,vol. 9, pp. 1319- 1327 ,(1990) , 10.1002/J.1460-2075.1990.TB08241.X
F. Oberhammer, J.W. Wilson, C. Dive, I.D. Morris, J.A. Hickman, A.E. Wakeling, P.R. Walker, M. Sikorska, Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. The EMBO Journal. ,vol. 12, pp. 3679- 3684 ,(1993) , 10.1002/J.1460-2075.1993.TB06042.X
D.G. Brown, X.M. Sun, G.M. Cohen, Dexamethasone-induced apoptosis involves cleavage of DNA to large fragments prior to internucleosomal fragmentation. Journal of Biological Chemistry. ,vol. 268, pp. 3037- 3039 ,(1993) , 10.1016/S0021-9258(18)53654-1
R C Duke, J J Cohen, Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death. Journal of Immunology. ,vol. 132, pp. 38- 42 ,(1984)
M Sikorska, J F Whitfield, P R Walker, T Youdale, C Smith, J Leblanc, Topoisomerase II-reactive chemotherapeutic drugs induce apoptosis in thymocytes. Cancer Research. ,vol. 51, pp. 1078- 1085 ,(1991)
G M Cohen, X M Sun, R T Snowden, D Dinsdale, D N Skilleter, Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochemical Journal. ,vol. 286, pp. 331- 334 ,(1992) , 10.1042/BJ2860331
C M Chresta, C Dive, J A Hickman, A Alejo-Herberg, A K Larsen, A Skladanowski, H M Beere, Investigation of the mechanism of higher order chromatin fragmentation observed in drug-induced apoptosis. Molecular Pharmacology. ,vol. 47, pp. 986- 996 ,(1995)
Sunderman Fw, The influence of zinc on apoptosis Annals of Clinical and Laboratory Science. ,vol. 25, pp. 134- 142 ,(1995)
Franziska Oberhammer, Gertraud Froschl, Roman Tiefenbacher, Salmaan H. Inayat-Hussain, Kelvin Cain, Helga Stopper, Hepatocyte death following transforming growth factor‐β1 addition Microscopy Research and Technique. ,vol. 34, pp. 247- 258 ,(1996) , 10.1002/(SICI)1097-0029(19960615)34:3<247::AID-JEMT7>3.0.CO;2-M
T Patel, S F Bronk, G J Gores, Increases of intracellular magnesium promote glycodeoxycholate-induced apoptosis in rat hepatocytes. Journal of Clinical Investigation. ,vol. 94, pp. 2183- 2192 ,(1994) , 10.1172/JCI117579