Relationship between genotype and phenotype of flagellin C in Salmonella

摘要: AIM: To discover the relationship between genotype and antigen serotype of flagellin C among Salmonella strains. METHODS: Fragment in plasmid pLS408 was cloned, sequenced compared with corresponding sequence other strains. strains including two typhi strains, one paratyphoid strain, enteritidis typhimurium strain were isolated from outpatients. Genome DNA purified respectively these clinical isolates, then fragment amplified by polymerase chain reaction, amplification products analyzed agarose gel electrophoresis. RESULTS: The cloned includes 582 nucleotides encoding variable region partial conservative pLS408. With comparison to sequences reported previously, there is only a little difference same flagellar both nucleotide amino acid level. Specific PCR H-1-d S. muenchen, typhimurium, but not or strains. CONCLUSION: In this experiment, specificity could be found central regions as it exists serotypes Salmonella. It may helpful developing rapid, sensitive, accurate PCR-based method detect H-1-d.