Glial cells assemble hyaluronan-based pericellular matrices in vitro.

摘要: The extracellular matrix (ECM) of the brain contains hyaluronan and proteoglycans, as does ECM cartilage. Aggrecan, major proteoglycan cartilage, forms large aggregates with hyaluronan, which then associate chondrocyte cell surface through an interaction binding proteins. In culture, chondrocytes elaborate hyaluronan-proteoglycan aggregates, form hydrated pericellular matrices (PCMs) that can be visualized by a particle exclusion assay (Knudson Toole: Dev Biol 112:308, 1985). It has recently been demonstrated embryonic glial cells also PCMs in culture (Deyst Brain Res 28:351, 1995). We demonstrate here different classes types endogenous culture. Less differentiated cells, evidenced their immunoreactivity for nestin, larger endogenously produced than astrocytes, defined GFAP. This vitro result may reflection volume space present mature brain. show further incorporate cartilage aggrecan into PCMs, both aggrecan-supplemented are dependent on hyaluronan. contrast, primary neurons from newborn (P0) P1 rat cortex neither express nor assemble exogenous hyaluronan/aggrecan PCMs. These results suggest immature not have ability to hyaluronan-based they raise possibility neural proteoglycans neuronal surfaces mechanism directly involve GLIA 20:193–202, 1997. © 1997 Wiley-Liss, Inc.