Quantification of oltipraz using liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study in rat plasma

摘要: Abstract An assay method for the determination of oltipraz, a candidate drug treatment liver fibrosis and cirrhosis, was developed in rat plasma using fast-flow protein precipitation (FF-PPT) coupled with LC–MS/MS quantification to reduce labor improve speed analysis. The applicability pharmacokinetic studies also evaluated. Oltipraz ethyl-oltipraz, an internal standard (IS), were analyzed by multiple reaction monitoring (MRM) at m / z transitions 227 → 193 241 → 174, respectively. A lower limit (LLOQ) 20 ng/mL observed, linear dynamic range from 20 4000 ng/mL ( R  > 0.997). accuracy, precision, dilution, recovery, stability deemed acceptable according FDA guidelines. concentrations measured successfully samples up 12 h post-dose rats that had received oral dose 60 mg/kg. findings indicate is rapid sensitive showing pharmacokinetics (PK) oltipraz other small animals, including rats.