Plasma deproteinization by precipitation and filtration in the 96-well format.

摘要: Abstract The need for fast bioanalytical methods within the pharmaceutical sector is rapidly growing. Sample preparation often bottleneck step. A new approach to increasing sample throughput involves precipitated protein removal by filtration in 96-well format, thereby eliminating centrifugation and manual handling of individual tubes. potential such a technique has been investigated determination an iron chelator, highly protein-bound compound (≥99.5%) plasma. An analog was used as internal standard. Acetonitrile plasma were sequentially aspirated, separated air gap, using electronic pipettor. They then dispensed into channel Empore™ filter PPT plate above filter, slight vacuum applied. eluate collected diluted prior injection. compounds reversed-phase chromatography detected UV at 295 nm. chromatographic run time 6 min. mean recovery following precipitation 78%, which shows that can apply compound. Replicate quality control samples prepared drug-free normal human four different concentrations. accuracy ranged from 87 108% with CV ranging 3 8%. described procedure simple, reproducible. It requires minimal equipment. required prepare manually only about 20 use 12-channel repeater pipettors reduces risk error improves productivity. Automation should be aid further when more than one day prepared.