Functionalized soluble nanopolymers for phosphoproteome analysis.

摘要: Protein phosphorylation plays a critical role in the regulation of many cellular functions. Phosphoproteomic analyses facilitate an in-depth understanding such phosphorylation-dependent signaling networks. The use mass spectrometry phosphoproteomics has been especially successful, but approach largely depends on efficient method to enrich phosphopeptides from complex mixtures. We have developed novel, effective soluble nanopolymer-based phosphopeptide enrichment technique, termed PolyMAC (polymer-based metal ion affinity capture). homogenous, hyperfunctional nature reagent makes it more competent choice for highly binding and isolation, which was demonstrated through several applications with simple protein mixture cell extract.