Identification of two candidate innate immune genes by transcriptional profiling and RNA interference in mouse mammary gland epithelial cells stimulated with lipopolysaccharide.

摘要: Mammary epithelial cells (MECs) play an important role in immune responses and inflammatory diseases such as mastitis, which is mainly attributed to the activation of Toll-like receptors release cytokines. However, overall change gene expression biological pathways MECs microbial factors stimulation remains unknown. Here, we analyzed profile mouse treated with lipopolysaccharide (LPS) for 24 h. Microarray analysis revealed that about 1548 genes differentially expressed, these involved 346 ontology terms 128 molecular pathways, particularly, some innate immune-associated were significant. By analyzing data pathway relation network, prioritized expressed respect LPS. The importance changes, indicating RNA interference-mediated inhibition two identified this analysis, transforming growth factor beta 1 (Tgf-β1) platelet-derived B (Pdgfb), reduced interleukin (IL)-6 tumor necrosis α production respectively, was verified. These findings delineate response patterns induced by LPS identify Tgf-β1 Pdgfb have been closely related immunity.