Host deficiency in ephrin-A1 inhibits breast cancer metastasis.

摘要: Background: The conventional dogma of treating cancer by focusing on the elimination tumor cells has been recently refined to include consideration microenvironment, which includes host stromal cells. Ephrin-A1, a cell surface protein involved in adhesion and migration, shown be suppressive context cell. However, its role not fully investigated. Here, we examine how ephrin-A1 deficiency affects growth metastasis murine model breast cancer. Methods: 4T1 were orthotopically implanted into mammary fat pads or injected tail veins wild-type ( Efna1 +/+ ), heterozygous +/- knockout -/- ) mice. Tumor growth, lung metastasis, recurrence after surgical resection measured. Flow cytometry immunohistochemistry (IHC) used analyze various populations primary tumors tumor-bearing lungs. Results: While did differ between , mice, significantly decreased mice had reduced colonization compared littermate controls as early 24 hours vein injection. Furthermore, established lesions proliferation those controls. Conclusions: Our studies demonstrate that does impact but affect providing less favorable metastatic niche for growth. Elucidating mechanisms impacts relapse may shed new light novel therapeutic strategies.