Sequence-specific Single-molecule Analysis of 8-Oxo-7,8-dihydroguanine Lesions in DNA based on Unzipping Kinetics of Complementary Probes in Ion Channel Recordings
作者： Anna E. P. Schibel , Aaron M. Fleming , Qian Jin , Na An , Jin Liu
DOI: 10.1021/JA205653V
关键词:
摘要: Translocation measurements of intact DNA strands with the ion channel α-hemolysin (α-HL) are limited to single-stranded (ssDNA) experiments as dimensions prevent double-stranded (dsDNA) translocation; however, if a short oligodeoxynucleotide is used interrogate longer ssDNA strand, it possible unzip duplex region when captured in α-HL vestibule, allowing strand translocate through channel. This unzipping process has characteristic duration based on stability duplex. Here, recordings detect presence and relative location oxidized damage site 8-oxo-7,8-dihydroguanine (OG) sequence-specific manner. OG engages base pairing C or A unique stabilities native Watson–Crick pairings, this phenomenon here engineer probe sequences (10–15mers) that, base-paired 65mer sequence interest, containing either G at single site, produc...
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