Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages
作者： H. Cao , R. J. Zhou , Q. W. Wei , C. J. Li , J. F. Gui
DOI: 10.1111/J.1439-0426.2010.01665.X
关键词:
摘要: Chinese sturgeon (Acipenser sinensis Gray) has a fast growth rate starting in early larval stage. Likely, the is attributed to hormone (GH); an essential polypeptide required for and development of vertebrates. In this study, GH cDNA was cloned from pituitary library. The AsGH consists approximately 954 bp size including 16 5'-untranslated region 296 3'-untranslated region. open reading frame (642 bp) encodes 214 amino acids (aa), which represents precursor composed 25 aa signal peptide followed by 189 mature polypeptide. polyclonal antibody prepared vitro expressed protein. Western blot analysis showed constant expression monomeric form 13 embryo stages several stages. Interestingly, two dimeric bands were detected since day 3 hatched larvae. Our findings suggest that may play important physiological functions embryonic sturgeon.
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