Shift in steroidogenesis in the ovarian follicles of the goldfish (Carassius auratus) during gonadotropin-induced oocyte maturation

摘要: Both partially purified chum salmon gonadotropin and 17α-hydroxyprogesterone stimulated in vitro production of testosterone by postvitellogenic follicles goldfish (Carassius auratus). Chum further enhanced the conversion exogenously supplied to 17α, 20β-dihydroxy-4-pregnen-3-one. The increased medium concentrations 20β-dihydroxy-4-pregnen-3-one were associated with induction final oocyte maturation. The capacity produce steroids response exogenous was examined females at various stages maturation following administration human chorionic vivo combined elevation holding temperature. maximum obtained from initial controls. In contrast, 17α 20β-diOHprog very low controls markedly during (3–6 hr injection), followed a significant decrease collected 15 hr. Estradiol-17β any gonadotropin-induced maturation. These results suggest that shift biosynthetic pathway follicle secretion predominantly is prerequisite step for goldfish.