Variations in hepatic progesterone 21-hydroxylase activity reflect differences in the microsomal concentration of rabbit cytochrome P-450 1.
作者： Eric F. Johnson , Keith J. Griffin
DOI: 10.1016/0003-9861(85)90253-X
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摘要: Abstract A monoclonal antibody specific for cytochrome P-450 1 that extensively (>95%) inhibits the hepatic 21-hydroxylation of progesterone was used in a two-site immunoradiometric assay to estimate concentration microsomes prepared from 24 individual, untreated New Zealand White rabbits. The 21-hydroxylase activities these ranged 0.2 5.8 nmol min−1 mg microsomal protein−1. Scatchard analysis revealed similar slopes and thus apparent affinities between microsome samples varied >10-fold activity. maximal extent binding different preparations greater exhibiting high as compared low activity, suggesting level reflects content 1. Quantitation based on 125I-labeled sequestered sample by heterologous adsorbed wells microtiter plate. These results indicate varies
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