Characterization of a second gene product related to rabbit cytochrome P-450 1.

摘要: A cDNA, p1-88, was cloned from a library constructed using rabbit liver mRNA. Sequence analysis indicates that p1-88 is highly similar (congruent to 95%) the p1-8, encodes cytochrome P-450 1 and had been isolated same library. The predicted amino acid sequence of protein encoded by IIC4, differs at 25 487 acids p1-8. IIC4 synthesized in vitro reticulocyte lysate primed with RNA transcribed coding bacteriophage T7 polymerase/promoter system. reacts two monoclonal antibodies recognize exhibits relative electrophoretic mobility as 1. In contrast, reactivity third antibody recognizing 1, 1F11, toward greatly diminished. latter extensively inhibits hepatic progesterone 21-hydroxylase activity recognizes phenotypic differences among rabbits microsomal concentration This difference immunoreactivity 1F11 suggests does not contribute significantly activity. S1 nuclease mapping demonstrates expression mRNAs corresponding are expressed equivalent extents exhibiting high low Thus, its regulation, immunoreactivity, inference catalytic properties although sequences 95%).