Proteomics profiling of cholangiocarcinoma exosomes: A potential role of oncogenic protein transferring in cancer progression.

摘要: Cholangiocarcinoma (CCA), a common primary malignant tumor of bile duct epithelia, is highly prevalent in Asian countries and unresponsive to chemotherapeutic drugs. Thus, newly recognized biological entity for early diagnosis treatment needed. Exosomes are small membrane bound vesicles found body fluids released by most cell types including cancer cells. The contain specific subset proteins nucleic acids corresponding play essential roles pathophysiological processes. present study aimed assess the protein profiles CCA-derived exosomes their potential roles. We have isolated from CCA cells namely KKU-M213 KKU-100 derived Thai patients were investigated incubation with normal human cholangiocyte (H69) internalized into H69 had no effects on viability or proliferation host Interestingly, only induced migration invasion Proteomic analysis disclosed multiple related that not exosomes. Consistent profile, β-catenin reduced E-cadherin expressions Collectively, our results suggest direct cell-to-cell transfer oncogenic via exosomal pathway may be novel mechanism progression metastasis.