Molecular cloning of an attenuated chicken anaemia virus isolate following repeated cell culture passage.
作者： D. Todd , T. J. Connor , Viola M. Calvert , Julie L. Creelan , B. M. Meehan
DOI: 10.1080/03079459508419057
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摘要: Summary The pathogenicity of the Cux‐1 isolate chicken anaemia virus (CAV) was substantially reduced following large numbers (50 to 173) passages in MDCC‐MSBl cells. Restriction endonuclease analysis polymerase chain reaction (PCR)— amplified DNAs and recombinant plasmids containing DNA inserts specified by CAV that had been passaged 173 times, indicated population high‐passage genetically diverse. A 210‐base pair (bp) insertion, a 19‐bp sequence identical four repeated sequences are located putative non‐coding region genome shown have become established passage number 30. Individual isolates were selected from using cloning transfection methodologies varied their pathogenicities. One cloned isolate, designated 10, produced virtually no levels aplasia bone marrow thymus atrophy. The...
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