Demonstration of the mineralocorticoid hormone receptor and action in human leukemic cell lines.
作者： M Mirshahi , S Mirshahi , N Golestaneh , Z Mishal , C Nicolas
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摘要: We studied the expression of mineralocorticoid receptor (MCR), and amiloride-sensitive sodium channel (ASSC) regulated by MCR, in human leukemic cell lines. Cell extracts from TF1 (proerythroblastic), HEL (human erythroblastic leukemia) U937 (myeloblastic) line were positive for ASSC, as a 82 kDa band Western blots developed with aid polyclonal antibody raised against peptide QGLGKGDKREEQGL, corresponding to region 44-58 alpha subunit epithelial (ENaC) cloned rat colon, linked KLH. The MCR revealed single about 102 cells. immunofluorescent labelling all lines showed nucleocytoplasmic localization but ASSC was exclusively membrane-bound these results confirmed confocal microscopy. cells evident predicted 843 bp (234 amino acids) electrophoresis PCR product obtained after total RNA had been reverse transcribed then amplified using primers 5'-AGGCTACCACAGTCTCCCTG-3' 5'-GCAGTGTAAAATCTCCAGTC-3' (sense antisense, respectively). ENaC similarly 5'-CTGCCmATG GATGATGGT-3' (sense) 5'-GTTCAGCTCGAAGAAGA-3' (antisense) 520 bp. In both cases, 100% identity observed between sequences products compared those known sources. multiplication influenced antagonists (RU 26752, ZK 91587) targeted specificity this selectively reversed natural hormone aldosterone. These steroids also provoked chromatin condensation population. permit new novel possibilities understand pathobiology leukemia delineate sodium-water homeostasis nonepithelial
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