Conversion of a Ribozyme to a Deoxyribozyme through In Vitro Evolution
作者： Natasha Paul , Greg Springsteen , Gerald F. Joyce
DOI: 10.1016/J.CHEMBIOL.2006.01.007
关键词:
摘要: An RNA ligase ribozyme was converted to a corresponding deoxyribozyme through in vitro evolution. The prepared as DNA molecule of the same sequence, and had no detectable activity. A population randomized variants this constructed evolved perform ligation at rate similar that starting ribozyme. When an it Thus, evolutionary transition from enzyme represents switch, rather than broadening, chemical basis for catalytic function. This transfer both information function is relevant between two different genetic systems based on nucleic acid-like molecules, postulated have occurred during early history life Earth.
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